pGL4.19[lucCP/Neo]

The pGL4.19[luc2CP /Neo] Vector(a–e) encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.19[luc2CP /Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 
0061 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT 
0121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC 
0181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC 
0241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 
0301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG 
0361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC 
0421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC 
0481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA 
0541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 
0601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC 
0661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC 
0721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC 
0781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC 
0841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 
0901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG 
0961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC 
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG 
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC 
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC 
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT 
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC 
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC 
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG 
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA 
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC 
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG 
1741 ATCGCCGTGA ATTCTGCTTG CAAGAACTGG TTCAGTAGCT TAAGCCACTT TGTGATCCAC 
1801 CTTAACAGCC ACGGCTTCCC TCCCGAGGTG GAGGAGCAGG CCGCCGGCAC CCTGCCCATG 
1861 AGCTGCGCCC AGGAGAGCGG CATGGATAGA CACCCTGCTG CTTGCGCCAG CGCCAGGATC 
1921 AACGTCTAAC TGCAGTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT 
1981 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 
2041 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 
2101 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA 
2161 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG TATTGGGCGC 
2221 TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT 
2281 AGCTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA 
2341 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 
2401 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 
2461 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 
2521 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 
2581 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT 
2641 TTGCAAAAAG CTCGATTCTT CTGACACTAG CGCCACCATG ATCGAACAAG ACGGCCTCCA 
2701 TGCTGGCAGT CCCGCAGCTT GGGTCGAACG CTTGTTCGGG TACGACTGGG CCCAGCAGAC 
2761 CATCGGATGT AGCGATGCGG CCGTGTTCCG TCTAAGCGCT CAAGGCCGGC CCGTGCTGTT 
2821 CGTGAAGACC GACCTGAGCG GCGCCCTGAA CGAGCTTCAA GACGAGGCTG CCCGCCTGAG 
2881 CTGGCTGGCC ACCACCGGCG TACCCTGCGC CGCTGTGTTG GATGTTGTGA CCGAAGCCGG 
2941 CCGGGACTGG CTGCTGCTGG GCGAGGTCCC TGGCCAGGAT CTGCTGAGCA GCCACCTTGC 
3001 CCCCGCTGAG AAGGTTTCTA TCATGGCCGA TGCAATGCGG CGCCTGCACA CCCTGGACCC 
3061 CGCTACCTGC CCCTTCGACC ACCAGGCTAA GCATCGGATC GAGCGTGCTC GGACCCGCAT 
3121 GGAGGCCGGC CTGGTGGACC AGGACGACCT GGACGAGGAG CATCAGGGCC TGGCCCCCGC 
3181 TGAACTGTTC GCCCGACTGA AAGCCCGCAT GCCGGACGGT GAGGACCTGG TTGTCACACA 
3241 CGGAGATGCC TGCCTCCCTA ACATCATGGT CGAGAATGGC CGCTTCTCCG GCTTCATCGA 
3301 CTGCGGTCGC CTAGGAGTTG CCGACCGCTA CCAGGACATC GCCCTGGCCA CCCGCGACAT 
3361 CGCTGAGGAG CTTGGCGGCG AGTGGGCCGA CCGCTTCTTA GTCTTGTACG GCATCGCAGC 
3421 TCCCGACAGC CAGCGCATCG CCTTCTACCG CTTGCTCGAC GAGTTCTTTT AATGATCTAG 
3481 AACCGGTCAT GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT 
3541 GTGTGTTCGA ACTAGATGCT GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC 
3601 CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT 
3661 GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
3721 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
3781 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
3841 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
3901 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
3961 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
4021 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
4081 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
4141 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
4201 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
4261 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
4321 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
4381 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
4441 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
4501 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
4561 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
4621 GGTCTGACAG CGGCCGCAAA TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA 
4681 GGCACCGATC TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC TCCCCGTCGT 
4741 GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC AGCGCAGCAA TGATGCCGCG 
4801 AGAGCCGCGT TCACCGGCCC CCGATTTGTC AGCAATGAAC CAGCCAGCAG GGAGGGCCGA 
4861 GCGAAGAAGT GGTCCTGCTA CTTTGTCCGC CTCCATCCAG TCTATGAGCT GCTGTCGTGA 
4921 TGCTAGAGTA AGAAGTTCGC CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG 
4981 CATCGTGGTA TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT CCCAGCGGTC 
5041 AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA GTCAGCTCCT TAGGGCCTCC 
5101 GATCGTTGTC AGAAGTAAGT TGGCCGCGGT GTTGTCGCTC ATGGTAATGG CAGCACTACA 
5161 CAATTCTCTT ACCGTCATGC CATCCGTAAG ATGCTTTTCC GTGACCGGCG AGTACTCAAC 
5221 CAAGTCGTTT TGTGAGTAGT GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG 
5281 GGACAACACC GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA ATCGTTCTTC 
5341 GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC AGTTCGATAT AGCCCACTCT 
5401 TGCACCCAGT TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCGGGGT GTGCAAAAAC 
5461 AGGCAAGCAA AATGCCGCAA AGAAGGGAAT GAGTGCGACA CGAAAATGTT GGATGCTCAT 
5521 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG 
5581 ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA ATATCTTTAT 
5641 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGTACTAACA TACGCTCTCC 
5701 ATCAAAACAA AACGAAACAA AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG 
5761 GTGCCAGAAC ATTTCTCT